Serum immunoglobulin G, M and A response to Cryptosporidium parvum in Cryptosporidium-HIV co-infected patients
Cryptosporidium parvum, the protozoan parasite, causes a significant enteric disease in immunocompromised hosts such as HIV patients. The present study was aimed to compare serum IgG, IgM and IgA responses to crude soluble antigen of C. parvum in HIV seropositive and seronegative patients co-infected with Cryptosporidium and to correlate the responses with symptomatology.
Methods: Cryptosporidium parvum specific serum antibody (IgG, IgM and IgA) responses were assessed by ELISA in 11 HIV seropositive Cryptosporidium positive (Group I), 20 HIV seropositive Cryptosporidium negative (Group II), 10 HIV seronegative Cryptosporidium positive (Group III), 20 HIV seronegative Cryptosporidium negative healthy individuals (Group IV) and 25 patients with other parasitic diseases (Group V).
Results: A positive IgG and IgA antibody response was observed in significantly higher number of Cryptosporidium infected individuals (Gp I and III) compared to Cryptosporidium un-infected individuals (Gp II, IV and V) irrespective of HIV/immune status. Sensitivity of IgG ELISA in our study was found to be higher as compared to IgM and IgA ELISA.
The number of patients with positive IgG, IgM and IgA response was not significantly different in HIV seropositive Cryptosporidium positive patients with diarrhoea when compared to patients without diarrhoea and in patients with CD4 counts <200 when compared to patients with CD4 counts >200 cells/ul.
Conclusions: The study showed specific serum IgG and IgA production in patients infected with Cryptosporidium, both HIV seropositive and seronegative as compared to uninfected subjects suggesting induction of Cryptosporidium specific humoral immune response in infected subjects. However, there was no difference in number of patients with positive response in HIV seropositive or seronegative groups indicating that HIV status may not be playing significant role in modulation of Cryptosporidium specific antibody responses.
The number of patients with positive IgG, IgM and IgA response was not significantly different in patients with or without history of diarrhoea thereby indicating that Cryptosporidium specific antibody responses may not be necessarily associated with protection from symptomatology.
Author: Kirti KaushikSumeeta KhuranaAjay WanchuNancy Malla
Credits/Source: BMC Infectious Diseases 2009, 9:179
Craig Fox of the Finger Lake Times
The British call them the "wet activities," which apparently refers to just about anything that can be done in a swimming pool.
Cryptosporidium is one of the most common enteric protozoan parasites of vertebrates with a wide host range that includes humans and domestic animals. It is a significant cause of diarrhoeal disease and an ubiquitous contaminant of water which serves as an excellent vehicle for transmission. A better understanding of the development and life cycle of Cryptosporidium, and new insights into its phylogenetic relationships, have illustrated the need to re-evaluate many aspects of the biology of Cryptosporidium. This has been reinforced by information obtained from the recent successful Cryptosporidium genome sequencing project, which has emphasised the uniqueness of this organism in terms of its parasite life style and evolutionary biology. This chapter provides an up to date review of the biology, biochemistry and host parasite relationships of Cryptosporidium.
The CDC issues similar warnings every year for public pools, but this is the first time they are focusing on homeowners and asking them to be aware of the dangers. RWI's include gastrointestinal, skin, ear, respiratory, eye, neurological and wound infections. The most commonly reported RWI is diarrhea and it is often caused when children swallow contaminated or dirty water while swimming.
Neenah officials billed the fountain as an interactive water feature when it was built in 2005.
Portland's brewmasters say its wrong for the federal Environmental Protection Agency to apply one standard to all open drinking water systems across the entire United States.
The Bull Run Watershed enjoys unique federal legislative protections. The Bull Run is a forested and undeveloped watershed on federal land, with restrictions on logging and entry of human and domestic animals (sources of viruses, bacteria and protozoa). These stringent watershed protections, some in place for over one hundred years and some legislated as recently as 2001, have increasingly been recognized by the EPA and the drinking water industry as the most cost effective strategy for protection of superb water quality. There have never been any demonstrated outbreaks of cryptosporidium associated with Bull Run water. LT2 (the filtration rule) completely disregards the effectiveness of a closed, pristine, water source like Bull Run in preventing disease outbreaks.
In Virginia, the Rye Valley Water Authority is being accused of going almost a year before telling the public that its water had gone bad. The Bristol Herald Courier reports on how that's got the whole community of Sugar Grove up in arms.